: In addition to Mysterio, the volume features confrontations with Mister Negative , Morbius , and a new iteration of the Vulture .

This volume collects the following US issues, originally published in 2010: Web of Spider-Man (Vol. 2) #4–5 Key Storyline: "The Gauntlet" (El Desafío)

The volume features a star-studded lineup of "Web-Heads" from the "Brand New Day" era: