2-(p-Hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzyl-7H-imidazo[1,2-a]pyrazin-3-one.

It serves as a substrate for various luciferases, such as Renilla luciferase (Rluc) and Gaussia luciferase (Gluc), to monitor gene expression.

Combined with the protein aequorin, it acts as a sensitive indicator for measuring intracellular calcium levels.

Used to study protein-protein interactions in living cells.

Generally stored at -20°C or -80°C, protected from light and oxygen to prevent degradation.